Gibco™ Advanced DMEM/F-12

Description

• Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation
• Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology
• Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat
• This Advanced DMEM/F-12 is manufactured as follows: With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red Without: L-glutamine, HEPES The complete formulation is available
• Using Advanced DMEM/F-12 Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride
• Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement
• Many cell lines do not require adaptation to this media
• The FBS concentration must be optimized for each cell line to obtain maximum serum reduction
• Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Order Info Shipping Condition: Room Temperature