Gibco™ FreeStyle™ F17 Expression Medium

Description

• FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation
• This is a multi-platform medium compatible with a range of transient expression systems
• The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use
• Gibco™ FreeStyle™ F17 Expression Medium features: Little or no required adaptation Ability to support suspension growth and transfection of 293-F and CHO cells Animal origin–free, protein-free, and chemically defined formulation Scalability in spinner flasks and bioreactors Little or no required adaptation Gibco™ 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco™ FreeStyle™ F17 Medium without adaptation
• Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ FreeStyle™ F17 Medium, usually without any further adaptation
• Cells usually require adaptation from serum-containing formulations
• Ability to support suspension growth and transfection of 293-F and CHO cells Gibco™ FreeStyle™ F17 Medium provides equal or better performance compared to Gibco™ FreeStyle™ 293 Expression Medium and Gibco™ FreeStyle™ CHO Medium
• Gibco™ FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 10 6 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 10 6 viable cells per mL
• Cells cultured in Gibco™ FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent
• Protein expression typically exceeds 75–150μg/mL IgG in 293-F cells
• Animal origin–free, protein-free, and chemically defined formulation Gibco™ FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
• Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition
• Scalability in spinner flasks and bioreactors Protein production using Gibco™ FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors
• The appropriate spinner or impeller speed and seeding density should be optimized for each system
• Depending on the impeller design and speed, it may be necessary to supplement Gibco™ FreeStyle™ F17 Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture
• cGMP manufacturing and quality system Gibco™ FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.