Gibco™ MEM α, nucleosides, no ascorbic acid

Description

• MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells
• MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells
• We offer a variety of MEM α modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This MEM α is modified as follows: With: Deoxyribonucleosides, Ribonucleosides, L-glutamine, Phenol Red Without: Ascorbic acid The complete formulation is available
• Using MEM α MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B 12 , biotin, and ascorbic acid
• MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells
• This MEM is modified without ascorbic acid
• MEM α contains no proteins, lipids, or growth factors
• Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• Order Info Shipping Condition: Room Temperature Compliance MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.