Gibco™ SILAC DMEM Flex Media, no glucose, no phenol red

Description

• SILAC DMEM Flex is Dulbecco’s Modified Eagle Medium (DMEM) without L-glutamine, L-lysine, and L-arginine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine
• DMEM is a widely used basal medium for supporting the growth of many different mammalian cells
• Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
• We offer a variety of Gibco™ DMEM modifications for a range of applications
• Find the right modification using the media selector tool
• DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
• SILAC DMEM Flex has been further modified to not contain lysine, arginine, and glutamine
• DMEM contains no proteins, lipids, or growth factors
• Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS
• DMEM uses a sodium bicarbonate buffer system (3.7 g/L), so requires a 5-10% CO 2 environment to maintain physiological pH
• cGMP Manufacturing and Quality System SILAC DMEM Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York
• The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.