Gibco™ StemScale™ PSC Suspension Medium

Description

• Gibco StemScale PSC Suspension Medium's simplified medium exchange and passaging workflows offers scalability in different formats and flexibility in feeding schedule
• Pluripotent stem cells expanded in StemScale medium maintain pluripotency and normal karyotype across multiple passages and can be differentiated to the three germ layers
• Maximal expansion potential Pluripotent stem cells cultured in StemScale PSC Suspension Medium consistently achieve 5-10 fold expansion per passage and three times the expansion capability versus other PSC suspension media
• Long term expansion of PSCs in StemScale medium (>30 passages) can be accomplished without loss of expansion potential, pluripotency, or genomic stability
• Superior workflow StemScale PSC Suspension Medium promotes the highly efficient self-aggregation of PSCs into spheroids, eliminating the need for microcarriers
• Unlike other self-assembly methods that require batch feeding, PSCs maintained in StemScale medium are fed by a 50% medium replacement strategy
• This method reduces metabolic waste in the culture while maintaining constant media volumes, important for maintenance of spheroid size
• The use of cell strainers during passaging, which can lead to cell loss, is not required and contributes to the ability to scale into larger vessel sizes for workflow scale-up
• Compatible with multiple vessel types Pluripotent stem cells can be expanded in StemScale medium across multiple vessel types, including 6-well plates, shaker flasks, and small-scale bioreactors, allowing users to scale PSC production as needed without sacrificing performance
• Flexible feeding schedule StemScale medium offers a flexible workflow that allows users to selectively determine when to passage cultures
• Feeding days can be skipped, providing the option of a weekend-free passaging schedule.