Description

• General description: High purity Fmoc-protected amino acid for research and process production of peptides, with very low levels of dipeptide, free-amino acids and acetic acid impurities. Fmoc-Gln(Trt)-OH has good solubility properties in most organic solvents, and its use has been shown to result in significantly purer peptides than other derivatives used for the introduction of Gln [1]. Coupling can be performed by standard procedures. The trityl-protecting group is normally removed by 95% TFA in 1-3 hours, with no alkylation of Trp. Associated Protocols and Technical ArticlesFmoc-amino acids for Peptide ProductionCleavage and Deprotection Protocols for Fmoc SPPSLiterature references[1] P. Sieber, et al. (1991) Tetrahedron Lett., 32, 739
• Application: Substrate specificity profiling of SARS-CoV-2 Mpro protease provides basis for anti-COVID-19 drug design: This study highlights the use of Fmoc-Gln(Trt)-OH in the synthesis of peptide substrates for biochemical assays, contributing to the development of COVID-19 therapeutics (Rut et al., 2020).
• Linkage: Replaces: 04-12-1090
• Analysis Note: Colour (visual): white to off whiteAppearance of substance (visual): powderColour index (0,5 M in DMF): ≤ 160 HazenIdentity (IR): passes testEnantiomeric purity: ≥ 99.8 % (a/a)Purity (HPLC): ≥ 99.0 % (a/a)Fmoc-ß-Ala-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-ß-Ala-Gln (Trt) -OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Gln(Trt)-Gln(Trt)-OH (HPLC): ≤ 0.1 % (a/a)Fmoc-Gln-OH (HPLC): ≤ 0.1 % (a/a)Assay free amino acid (GC): ≤ 0.2 %Purity (TLC(011A)): ≥ 98 %Purity (TLC(0811)): ≥ 98 %Solubility (25 mmole in 50 ml DMF): clearly solubleAssay (acidimetric): ≥ 93.0 %Water (K. F.): ≤ 2.0 %Ethyl acetate (HS-GC): ≤ 0.5 %Acetate (IC): ≤ 0.02 %To see the solvent systems used for TLC of Novabiochem® products please click here.
• Legal Information: Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany