54457

HEPES

Manufacturer: Sigma Aldrich

CAS Number: 7365-45-9

Synonym(S): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Select a Size

Pack Size SKU Availability Price
10 G 54457-10-G In Stock ₹ 5,461.20
50 G 54457-50-G In Stock ₹ 19,968.90
250 G 54457-250-G In Stock ₹ 70,751.40

54457 - 10 G

₹ 5,461.20

In Stock

Quantity

1

Base Price: ₹ 5,461.20

GST (18%): ₹ 983.016

Total Price: ₹ 6,444.216

product name

HEPES, BioUltra, for molecular biology, ≥99.5% (T)

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (T)

form

powder

impurities

DNases, none detected RNases, none detected insoluble matter, passes filter test phosphatases, none detected proteases, none detected

ign. residue

≤0.1% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H2O)

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Description

  • General description: HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]
  • Application: HEPES has been used as a component of:DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells[3]RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)[4]Recapture solution to re-capture RNA exosomes from the glycerol gradient[5]ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation[6]2X HBS buffer that is used in transfection process for the production of recombinant lentivectors[7]
  • Other Notes: Easily compare specifications for HEPES products with the HEPES specification table.

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves