H3537

HEPES solution

Manufacturer: Sigma Aldrich

CAS Number: 7365-45-9

Synonym(S): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Select a Size

Pack Size SKU Availability Price
100 ML H3537-100-ML In Stock ₹ 9,035.40
500 ML H3537-500-ML In Stock ₹ 17,526.90
1 L H3537-1-L In Stock ₹ 38,483.70

H3537 - 100 ML

₹ 9,035.40

In Stock

Quantity

1

Base Price: ₹ 9,035.40

GST (18%): ₹ 1,626.372

Total Price: ₹ 10,661.772

product name

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grade

BioPerformance Certified

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested DNase, RNase, Protease, Nickase, free endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

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Description

  • General description: HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]
  • Application: RNAse HEPES has been used:To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets[3]To recover purified ribonucleotide[4]As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing[5]As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets[6]

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable