H7523

HEPES

Manufacturer: Sigma Aldrich

CAS Number: 7365-45-9

Synonym(S): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Select a Size

Pack Size SKU Availability Price
50 G H7523-50-G In Stock ₹ 14,174.70
250 G H7523-250-G In Stock ₹ 43,545.30

H7523 - 50 G

₹ 14,174.70

In Stock

Quantity

1

Base Price: ₹ 14,174.70

GST (18%): ₹ 2,551.446

Total Price: ₹ 16,726.146

product line

BioXtra

Quality Level

400

Assay

≥99.5% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.1% (as SO4)

loss

≤1.0% loss on drying, 110°C

pH

5.0-6.5 (1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

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Description

  • General description: HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]
  • Application: HEPES has been used:As a component of homogenisation buffer used in protein sample preparation[3]To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation[4]As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation[5]As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.[6][7]
  • Other Notes: Easily compare specifications for HEPES products with the HEPES specification table.

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves