Description

• General description: MES Buffer, recognized as one of the "Good" buffers employed in biological and biochemical applications, plays a crucial role across various research domains. This zwitterionic and morpholinic buffer proves highly valuable due to its effective buffering capacity within the pH range of 5.5 to 6.7. Notably, MES Buffer does not engage in complex formation with the majority of metals commonly employed in environmental and biological studies. Its solubility in water is excellent, and it exhibits minimal lipid solubility, rendering it impermeable to membranes.This versatile buffer may find widespread application in maintaining a stable environment in solutions dedicated to cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate. MES Buffer also proves valuable for fine-tuning the pH of growth media, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.
• Application: It has been utilized as a buffer bicarbonate‐containing culture medium[1]
• Features and Benefits: Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applicationsEffective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)Highly soluble in waterMinimal metal ion bindingLess toxic to cells than other buffers such as Tris and phosphateStable in a wide pH rangeLow UV absorptivity and Minimal reactivity
• Quality: Low moisture
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