Description

• Application: L-α-Phosphatidylcholine is suitable for use:as a component of human erythroid massive amplification culture (HEMAdef) medium for clinical expansion of human erythroblasts[1]in the chloride efflux assay to examine the chloride permeability of lipid vesicles conferred by soluble CLIC1[2]in an assay to measure solubilized wax synthase activity[3]as a component of buffer A used for the resuspension of the cholate-solubilized catalytic unit of adenylate cyclase[4]as a cryoprotectant for freezing ruminant sperm[5]as a substrate for the estimation of phospholipase activity[6]to make liposomes to assess their efficiency of recruitment of ARF (ADP ribosylation factor) and AP-1 (adaptor protein)[7]in the preparation of the isolated catalytic unit of soluble adenylate cyclase[8]
• Biochem/physiol Actions: Phosphatidylcholine is the major membrane phospholipid in eukaryotic cells, forming the structural component of these membranes. It also serves as a reservoir for several lipid messengers and a source for several bioactive lipids such as, lysophosphatidylcholine, phosphatidic acid, diacylglycerol, lysophosphatidylcholine, platelet activating factor, and arachidonic acid.[9]
• Preparation Note: Purified from P 5638