Description

• General description: By contrast to typical PEG reagents that contain heterogeneous mixtures of different PEG chain lengths, this PEG reagent is a homogeneous compound of defined molecular weight and spacer arm length, providing greater precision in optimization and characterization of crosslinking applications. Homobifunctional crosslinkers containing N-hydroxysuccinimide (NHS) esters are often used for low-resolution 3-D studies of protein structure and protein interaction analysis.
• Features and Benefits: Reactive groups: NHS ester (both ends)Reactive towards: amino groups (primary amines)NHS esters react with—NH2 groups at pH 7-9, forming stable amide bondsWater-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)Membrane-impermeable, allowing for cell surface labelingIrreversibly crosslink proteins or peptides by flexible PEG spacer armsPolyethylene glycol spacer arms help maintain conjugate solubilityPure compound with defined structure and molecular weight, ensuring reproducible protein-modification effectsPEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicityIdeal for small molecule or peptide conjugations
• Caution: This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.