D9163

DL-Dithiothreitol

suitable for electrophoresis, ≥99%

Manufacturer: Sigma Aldrich

CAS Number: 3483-12-3

Synonym(S): threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

Select a Size

Pack Size SKU Availability Price
1 G D9163-1-G In Stock ₹ 9,504.35
5 G D9163-5-G In Stock ₹ 15,230.00
25 G D9163-25-G In Stock ₹ 36,260.00

D9163 - 1 G

₹ 9,504.35

In Stock

Quantity

1

Base Price: ₹ 9,504.35

GST (18%): ₹ 1,710.783

Total Price: ₹ 11,215.133

Quality Level

200

Assay

≥99%

form

powder

reaction suitability

reagent type: reductant

technique(s)

electrophoresis: suitable

mp

40-44 °C41-44 °C (lit.)

solubility

H2O: 50 mg/mL, clear, colorless to very faintly yellow

cation traces

heavy metals (as Pb): ≤10 ppm

absorption

≤0.04 at 280 at 0.02 M

storage temp.

2-8°C

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Description

  • Application: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
  • Analysis Note: Tested for use in reduced SDS-PAGE

SAFETY INFORMATION

Pictograms

GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H315,H318

Precautionary Statements

P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves