Description

• General description: Mass of glucose oxidase (GOX), a flavoprotein ranges from approximately 130 to 175 kDa.[1] Some fungi and insects are capable of producing GOX.[2]
• Application: Glucose Oxidase from Aspergillus niger has been used: in the (glucose oxidase) GO reagent to measure the glucose content by the glucose oxidase (GO) method[3]to activate the human renal carcinoma cell line for constructing the oxidative stress model[4]to study its influence in the paste on the analytical performance of the bioelectrode[5]
• Biochem/physiol Actions: Glucose oxidase (GOX) oxidizes D-glucose to D-gluconolactone and hydrogen peroxide. Hydrogen peroxide, the catalytic product of GOX, serves as an anti-bacterial and anti-fungal agent.[2] It is safe and can be used in several industrial applications like baking, dry egg powder production, wine production, gluconic acid production, etc. It possesses electrochemical activity, which makes it extremely important in glucose sensors and in fuel cell applications.[2]
• Quality: May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
• Unit Definition: One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.
• Analysis Note: Protein determined by biuret