4591510
Cytiva rProtein A Sepharose™ Fast Flow Affinity Media
Designed for purification of monoclonal and polyclonal antibodies at labs and process scales
Manufacturer: Fischer Scientific
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For Use With (Application)
For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Storage Requirements
2°C to 8°C
Flow Rate
150 to 250 cm/Hoμr
Height (English) Bed
9.84 in.
Max. Pressure
100 kPa
pH Range
2 to 9
Ligand Type
Recombinant Protein A (E. coli)
Stability
Stable in aqueous Buffers commonly used in Protein A Chromatography
Product Type
rProtein A Sepharose Fast Flow Media
Particle Size
60 to 165 μm
Height (Metric) Bed
25 cm
Matrix
4% Cross-Linked Agarose
Storage Buffer
20% Ethanol
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Description
- Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage No mammalian components involved during manufacturing process Matrix: 4% cross-linked agarose Average particle size: 90μm (d50V) Ligand: 6mg rProtein A/mL medium ( E
- coli ) Binding capacity: ∽50mg human IgG/mL media pH stability working range: 3 to 10 Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed