Invitrogen™ FirstChoice™ RLM-RACE Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1700-Each-of-1 | In Stock | ₹ 73,752.72 |
AM1700 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 73,752.72
GST (18%): ₹ 13,275.49
Total Price: ₹ 87,028.21
Product Type
RLM-RACE Kit
Content And Storage
The following components should be stored at –20°C:Calf Intestine Alkaline Phosphatase10X CIP BufferTobacco Acid Pyrophosphatase10X TAP Buffer, T4 RNA Ligase10X T4 RNA Ligase Buffer5' RACE Adapter3' RACE Adapter5' RACE Outer Primer5' RACE Inner Primer3' RACE Outer Primer3' RACE Inner PrimerAmmonium Acetate Stop SolutionM-MLV Reverse TranscriptasedNTP Mix10X RT Buffer10X PCR BufferRandom DecamersRNase InhibitorMouse Thymus RNA5' RACE Outer Control Primer5' RACE Inner Control Primer5' PCR Control Primer3' RACE Control PrimerNuclease-free water may be stored at any temperature.
Optimal Reaction Temperature
42°C
Shipping Condition
Dry Ice
Product Line
Ambion™, FirstChoice™
Quantity
1 Kit
Includes
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature.
Final Product Type
cDNA
Reverse Transcriptase
M-MLV
Format
Kit
For Use With (Application)
cDNA Libraries & Library Construction
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Description
- Description The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol
- The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages
- From RNA to PCR product in less than a day Yields single, specific product from rare transcripts Selects 5' and/or 3' ends of true messages Efficient— Al enzymatic reactions are optimized to ensure detection of even rarest mRNA Final product size: 7kb or less Optimal reaction temperature: 42°C Sample: RNA Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages Improvement to basic RACE protocol Selects full-length mRNA—no rRNA, tRNA or degraded mRNA Optimal reaction temperature: 42°C Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction Order Info Shipping Condition: Dry ice