K400
R&D Systems™ BostonBiochem UbiCREST Enzyme Set
Manufacturer: R&D Systems™
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Content And Storage
Store the unopened product at -70° C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Quantity
1 Kit
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Description
- UbiCREST (Ubiquitin Chain Restriction Analysis) is designed to analyze the ubiquitin linkages present in free polyubiquitin chains or polyubiquitin chains conjugated to a substrate protein
- Developed in the Komander lab, the UbiCREST approach utilizes the linkage specificity of the ovarian tumor domain (OTU) class of deubiquitinases (DUBs) to characterize the linkages present in a sample containing polyubiquitin
- This kit provides DUBs that are useful for in vitro analysis of user supplied polyubiquitinated protein samples
- The samples may be generated by in vitro ubiquitination reactions, or obtained from cell lysates or other complex mixtures following immunoprecipitation or other means of purification.