4521356
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Manufacturer: Fischer Scientific
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Concentration
5 U/μL
Polymerase
DNA Polymerase I
Compatible Buffer
Restriction Enzyme, PCR, RT Buffer
Product Type
Klenow Fragment
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Description
- Description Klenow Fragment, exo-, is the large fragment of DNA polymerase I
- It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I
- The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit
- Highlights Lacks 3'→5' exonuclease activity Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides) Active in restriction enzyme, PCR, and RT buffers