4918164

Invitrogen™ Ambion™ RNase I, cloned, 100 U/μL

Efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Concentration

100 U/μL

Shipping Condition

Dry Ice

Product Type

RNase I

Content And Storage

-20°C

Enzyme

RNase

Product Line

Ambion™

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Description

  • RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate
  • RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA
  • It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays
  • Supplied in one tube of 10,000U (100U/μL) Endonuclease-free, exonuclease-free, protease-free Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min
  • at 37°C DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting Order Info Shipping Conditions: Dry ice