4918181
Invitrogen™ Ribonuclease H
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA
Manufacturer: Fischer Scientific
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Concentration
2 U/μL
Shipping Condition
Approved for shipment on Wet or Dry Ice
Enzyme
RNase
Product Type
Ribonuclease H
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Description
- Source: Purified from E
- coli expressing the E
- coli RNase H gene on a plasmid Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease Unit definition: One unit hydrolyzes 1nmol of RNA in 3 H-labeled poly(A)poly(dT) to acid-soluble material in 20 min
- at 37°C Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl 2 , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol 3 H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min
- at 37°C Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription Order Info Shipping Conditions: Approved for shipment on wet or dry ice