MP Biomedicals™ Collagenase Type I fromClostridium histolyticum

Description

• The collagenase product is a mixture of enzymes secreted by C
• histolyticum , with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes
• The main components are two collagenases, clostripain, and a neutral protease
• The synergistic action of these enzymes degrade collagen and other intracellular material
• The action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue
• Various types of collagen are the natural substrates for collagenase
• Unit Definition: One unit will liberate peptides from collagen equivalent in ninhydrin color to 1.0μmol of L-leucine in 5 hr
• at pH 7.4 and 37°C in presence of calcium ions
• Contains clostripain, neutral protease and trypsin activities.