ICN15003650
MP Biomedicals™ Peroxidase From Horseradish
Manufacturer: MP Biomedicals™
The price for this product is unavailable. Please request a quote
Color
Brown
For Use With (Application)
ELISA, immunoblotting, immunohistochemistry
Source
Horseradish
Product Type
Peroxidase
Inhibitors
p-Aminobenzoic Acid, Azide, Cyclopropanone, L-cystine, Dichromate, Ethylenethiourea, Hydroxylamine, Sulfite and Vanadate and Divalent Metals Cd, Co, Cu, Fe, Mn, Ni, Pb
pH
7
Content And Storage
-20°C
Quantity
50 kU
Molecular Weight (g/mol)
44000
Form
Lyophilized Powder
Related Products
Description
- A plant glycohemoprotein, Horseradish Peroxidase's (HRP's) enzymatic activity is due to the cyclic reduction and oxidation of the iron atom in the hematin group
- The iron-containing hemin group is covalently bound to the glycoprotein apoenzyme
- HRP readily combines with hydrogen peroxide and the resultant complex can oxidize a wide variety of chromogenic hydrogen donors
- It is composed of seven isozymes, all containing photohemin IX as prosthetic group
- Specific activity: ≥60 purpurogallin units/mg Extinction coefficient: EmM = 100 (403 nm)(Lit.) Presentation: brown powder Isoelectric Point: 7.2(Lit.) Optimum pH: 7.0(Lit.) Unit definition: amount of enzyme causing the production of 1mg of purpurogallin in 20 seconds at 20°C RZ: ≥0.6 RZ Definition: The ratio of the absorbance at 403nm to the absorbance at 275nm
- This value is an expression of the ratio of hemin to protein content.