Thermo Scientific™Brilliance™ Cronobacter Sakazakii Agar (DFI formulation) (Dehydrated)

Description

• Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with Thermo Scientific™ Brilliance ™ Cronobacter Sakazakii Agar (Dehydrated)
• A chromogenic substrate present in the medium detects a-glucosidase reaction in Cronobacter sakazakii
• The enzyme a-glucosidase, present in Chronobacter sakazakii , hydrolyses the substrate producing blue-green colonies on this pale yellow medium
• Cronobacter sakazakii is a Gram-negative rod-shaped bacterium that rarely causes disease in healthy adults
• It has been implicated in outbreaks of disease in premature infants (neonates)
• Research suggests that neonates, or those infants who have other medical conditions, are more susceptible to this infection
• Most reported cases of infection are severe, including sepsis (bacteria in the blood, meningitis, or necrotizing enterocolitis (severe intestinal infection)
• Use Brilliance Cronobacter Sakazakii Agar for differentiation and enumeration of Cronobacter sakazakii from infant formula and other food samples
• Selective: due to the presence of desoxycholate, the medium is selective against most Gram-positive organisms
• Easy-to-read: Cronobacter sakazakii produces blue-green colonies on this medium
• Cronobacter sakazakii is an opportunistic pathogen which has been isolated at low levels from powdered infant formulas
• The organisms’ high tolerance to desiccation provides a competitive advantage for Cronobacter sakazakii in dry environments, as found in milk powder factories, and thereby increases the risk of post-pasteurization contamination of the finished product 1
• The current FDA method 1 for the detection of Cronobacter sakazakii is based on yellow pigment production and originated from the pioneering work of Muytjens et al
• 2
• Samples are incubated in water overnight then enriched in EE Broth (CM0317), followed by plating on VBRGA (CM0485) to isolate Enterobacteriaceae
• Five colonies are selected and streaked on Tryptone Soya Agar (CM0131), incubated for up to three days and observed for yellow colonies, typical of Cronobacter sakazakii
• However, this method does not select for Chronobacter sakazakii and the combined use of EE broth and VRBGA could allow other Enterobacteria to outgrow Chronobacter sakazakii and give false negative results
• It is not possible to select for Chronobacter sakazakii colonies from VRBGA plates on the basis of colony morphology as they will appear the same as other Enterobacteria.