Thermo Scientific™ Middlebrook 7H11 Agar (Dehydrated)

Description

• Isolate and cultivate Mycobacterium spp
• using Thermo Scientific™ Remel™ Middlebrook 7H11 Agar (Dehydrated)
• In 1958, Middlebrook and Cohn improved the first formulation of oleic acid and albumin agar to obtain 7H10 Agar, which allows faster and more luxuriant growth of Mycobacterium species 1
• In 1968, Cohn et al
• further modified the medium by adding casein hydrolysate, glycerol and OADC enrichment which stimulated the growth of other mycobacteria which could not grow on 7H10 agar 2
• Isolate and cultivate Mycobacterium spp
• using Middlebrook 7H11 Agar
• This medium is used for qualitative procedures
• This medium contains inorganic salts which are essential for the growth of mycobacteria
• Selective: Malachite-green dye present in the media inhibits bacteria other than Mycobacterium spp
• Allows luxuriant growth of Mycobacterium Casein hydrolysate serves as a growth stimulant for drug resistant strains of Mycobacterium tuberculosis 3
• Biotin helps to stimulate the growth of damaged mycobacteria
• Glycerol acts as a source of carbon and anergy while ODAC Enrichment provides nutrients required for the growth of mycobacteria
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.