Gibco™ CultureOne™ Supplement (100X)

Description

• CultureOne Supplement is a chemically-defined, serum-free supplement designed to significantly improve the differentiation of neural stem cells (NSCs) to neurons
• Compared to conventional differentiation methods where NSCs can overgrow and become burdensome, CultureOne Supplement eliminates more than 75% of contaminating neural progenitor cells with minimal cell death and no effect on other kinase-mediated pathways
• The resulting superior neuronal cell cultures of evenly distributed, differentiated neurons enable improved downstream assays, accelerated neuronal maturation, and seamless maintenance for five weeks or more
• Each vial of CultureOne Supplement contains a 100X 5-mL liquid reagent that can be added easily to any conventional neuronal differentiation medium, such as Neurobasal Medium with B-27 Supplements
• After two weeks of differentiation with CultureOne Supplement, the supplement can be removed and the resulting cultures will contain considerably fewer progenitor cells for five weeks or more
• Improved downstream assays Analysis of NSC-derived neurons can be difficult due to the presence of overgrown progenitor cells in the culture
• These invasive cells can make imaging and electrophysiology experiments challenging and call into question the physiological relevance of gene expression assays
• CultureOne Supplement reduces proliferating progenitor cells, giving you a cleaner and more relevant neuronal cell population, which significantly improves the assay experience and outcome
• Accelerated maturation of differentiated neurons With CultureOne Supplement, NSC-derived neurons express mature physiological markers faster and develop longer neurites than untreated NSC-derived neuronal cultures
• CultureOne Supplement-treated neurons also express significantly higher numbers of voltage gated calcium ion channels, which are an important marker for neural maturity and excitability
• Longer culture of NSC-derived neurons For experiments that require NSC-derived neurons to mature for long periods of time, CultureOne Supplement seamlessly enables maintenance of enriched, differentiated neurons for five weeks or longer
• By contrast, conventional differentiation methods result in proliferating neural progenitor cells, which tend to clump and cause the culture to peel off of the plate
• For established protocols using N-2 Supplement, access our Gibco Neurobiology Protocol Handbook
• View publications using Gibco neurobiology products at our Gibco CellCite .