Gibco™ Terrific Broth

Description

• Gibco Terrific Broth is a ready-to-use liquid growth medium used for bacterial culture
• Our filter-sterilized Terrific Broth can be used for nearly all research applications that require a liquid bacterial culturing step, such as protein expression, cloning, and expansion
• Terrific Broth is a nutritionally rich bacterial medium for higher density growth and maintenance of recombinant E
• coli strains
• It was specifically developed to achieve higher plasmid DNA yield from E
• coli and can yield 4–7 times more DNA from pUC-based vectors compared to LB medium
• This versitle broth is also useful for processing a large number of different clones from smaller cultures
• Terrific Broth formulation per liter 11.8 g Tryptone 23.6 g Yeast extract 9.4 g Dipotassium hydrogen phosphate 2.2 g Potassium dihydrogen phosphate 4 mL Glycerol (0.4%) • TB medium contains higher concentration of yeast extract compared to other media • Offers better buffering capacity due higher salt concentrations • Added glycerol has a stabilizing effect on recombinant proteins Primary applications • Growth of E
• coli strains to achieve higher plasmid yield • Expression of recombinant proteins using bacteria Gibco media bottles Each media bottle features an angled opening for simplified pipetting
• The 1 L Gibco media bottle uses 39% less source material than comparable media bottles on the market
• Both the cap and bottle can be recycled as they are made from highly recyclable high-density polyethylene (HDPE) and polyethylene terephthalate (PETE)
• Find the media format that fits your needs We offer a variety of ready-to-use media formulations, such as LB Broth (Cat
• No
• 10855021) or S.O.C recovery medium (Cat
• No
• 15544034).