Thermo Scientific™ Malonate Broth,Ewing

Description

• Differentiate members of Enterobacteriaceae based on sodium malonate utilization using Thermo Scientific™ Remel™ Malonate Broth (Ewing Modification)
• Malonate Broth is recommended to differentiate certain subgroups of Salmonella from other Enterobacteriaceae 1
• Leifson developed a liquid medium to differentiate Escherichia coli from Enterobacter spp 2
• Ewing et al
• modified the Leifson's formulation by adding a trace amount of dextrose and yeast extract to stimulate growth of certain organsims that fail to grow and obseved their reaction on malonate 3,4
• Differentiate members of Enterobacteriaceae based on sodium malonate utilization using Malonate Broth (Ewing Modification)
• Recommended to differentiate certain subgroups of Salmonella from other Enterobacteriaceae 1 Blue color development due to utilization of malonate is easy to interpret Ready to use – Convenience of prepared media An organism that utilizes sodium malonate as a carbon source and ammonia sulfate as a nitrogen source produces an alkaline reaction and organisms which cannot utilize malonate will ferment dextrose, causes the pH indicator to change from green to yellow thus producing an acid reaction.