Thermo Scientific™ Pierce™ Glutathione Coated Plates

Description

• Pierce Glutathione Coated Plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins
• These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system
• Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein
• As the plates are pre-blocked, initial purification of the cell lysates is not necessary
• Thermo Scientific Pierce Glutathione Coated Plates are microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins
• Features of Glutathione Coated Plates include: • Selective —specifically binds fusion proteins containing glutathione S-transferase • Capacity —binds approx
• 10 ng GST protein per well • Versatile —ready-to-bind plate format is useful for protein interaction studies • Compatible —detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates) • Ready to use —Pre-blocked with SuperBlock Blocking Buffer to reduce nonspecific binding