Thermo Scientific™ Pierce™ NeutrAvidin™ Coated Plates

Description

• Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule
• Thermo Scientific NeutrAvidin Protein is avidin that has been deglycosylated to prevent nonspecific lectin-binding
• With a near-neutral pI (6.3), this results in lower nonspecific interactions in certain applications compared with streptavidin
• NeutrAvidin Protein lacks the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells
• Thermo Scientific NeutrAvidin Protein Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays
• Features of NeutrAvidin Protein Coated Plates include: • White plates for chemiluminescent detection methods • Pre-blocked with Blocker BSA Buffer or SuperBlock Blocking Buffer • Easy and gentle immobilization of biotin-containing conjugates • Low nonspecific binding • No denaturing of the protein component of a conjugate upon binding • Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene • Less expensive than streptavidin coated plates • Lowest nonspecific binding properties of all biotin-binding proteins