B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

Manufacturer: Sigma Aldrich

CAS Number: 97753-82-7

Synonym(S): 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

Select a Size

Pack Size SKU Availability Price
100 MG B2904-100-MG In Stock ₹ 18,175.18
500 MG B2904-500-MG In Stock ₹ 60,717.43

B2904 - 100 MG

₹ 18,175.18

In Stock

Quantity

1

Base Price: ₹ 18,175.18

GST (18%): ₹ 3,271.532

Total Price: ₹ 21,446.712

grade

for molecular biology

Quality Level

200

sterility

non-sterile

Assay

≥98% (HPLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

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Description

  • General description: Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
  • Application: Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]
  • Principle: When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves