Yeast Nitrogen Base Without Amino Acids

Description

• General description: Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.
• Application: Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.
• Components: Component, amount (μg/L unless indicated)Nitrogen Sources:Ammonium sulfate, 5.0 g/LVitamins:Biotin, 2.0Calcium pantothenate, 400Folic acid, 2.0Inositol, 2.0 mg/LNicotinic acid, 400p-Aminobenzoic acid, 200Pyridoxine HCl, 400Riboflavin, 200Thiamine HCL, 400Trace Elements:Boric acid, 500Copper sulfate, 40Potassium iodide, 100Ferric chloride, 200Manganese sulfate, 400Sodium molybdate, 200Zinc sulfate, 400Salts:Potassium phosphate monobasic, 1.0 g/LMagnesium sulfate, 0.5 g/LSodium chloride, 0.1 g/LCalcium chloride, 0.1 g/L
• Preparation Note: 1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.2. Warm if necessary to solubilize and sterilize by filtration.Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.