Anti-Digoxigenin

Description

• General description: Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.[1]
• Specificity: The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
• Application: Anti-Digoxigenin has been used in DNA tethering.[3] It has been used to attach DNA molecule to the glass surface of the flow cell.[4]
• Biochem/physiol Actions: In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.[7]
• Preparation Note: Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: ELISA: for coating: 2 to 4 μg/mlImmunohistocytochemistry: 0.5 to 2 μg/mlIn situ hybridization: 0.2 to 0.4 μg/mlWestern blot: 0.5 to 2 μg/mlWorking solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
• Reconstitution: Add 1 ml PBS to a final concentration of 200 μg/ml.
• Analysis Note: No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.
• Other Notes: For life science research only. Not for use in diagnostic procedures.