11373242910
Fluorescein-12-dUTP
Manufacturer: Sigma Aldrich
Synonym(S): Fluorescein-12-dUTP, dUTP
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 μL | 11373242910-25-μL | In Stock | ₹ 28,069.23 |
11373242910 - 25 μL
In Stock
Quantity
1
Base Price: ₹ 28,069.23
GST (18%): ₹ 5,052.461
Total Price: ₹ 33,121.691
Quality Level
100
Assay
≥85% (HPLC)
form
solution
mol wt
Mr 1018.4 (fluorescein-12-dUTP-Li4)
packaging
pkg of 25 μL (25 nmol; 1mM)
manufacturer/tradename
Roche
technique(s)
ELISA: suitablehybridization: suitable
storage temp.
−20°C
Related Products
Description
- Application: Fluorescein-12-dUTP is used as a substrate for: terminal transferaseDNA polymerase I (holoenzyme and Klenow fragment),Taq DNA polymerasereverse transcriptase (e.g., from AMV and M-MuLV).Fluorescein-12-dUTP has been used to label nuclear extracts during PCR.[1] It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL.[2] Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection[3] or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.
- Quality: Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).
- Physical form: Fluorescein-12-dUTP, tetralithium salt.
- Storage and Stability: The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.
- Other Notes: For life science research only. Not for use in diagnostic procedures.
SAFETY INFORMATION
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash