MP Biomedicals™ GeneClean II Kit

Description

• The GENECLEAN™ II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions
• Glassmilk™ slurry allows flexibility of reacion size and volume
• The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling
• The GENECLEAN™ II Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels
• This kit can also be used to purify DNA from polyacrylamide gels
• Typical recovery of DNA is up to 80%
• Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice
• The GENECLEAN™ II Kit combines silica-binding technology with the convenience of a spin or vacuum column format
• DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane
• Contaminants are removed by a simple spin or vacuum wash
• The bound DNA is then eluted
• DNA fragments of interest are extracted by solubilizing slices of an agarose gel
• The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer
• The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution
• Contaminants are removed by a simple spin or vacuum wash
• Finally, the bound DNA is eluted in Tris buffer.