Invitrogen™ MagMAX™ DNA Multi-Sample Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 44-130-20-Each-of-1 | In Stock | ₹ 25,154.64 |
44-130-20 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 25,154.64
GST (18%): ₹ 4,527.835
Total Price: ₹ 29,682.475
Content And Storage
Contains:• DNA Binding Beads, 10 mg/mL, 1 x 1.8 mL (same for both kits). Store at 2 to 8 °C• DNA Elution Buffer 1, 1 x 15 mL (same for both kits). Store at RT• DNA Elution Buffer 2, 1 x 15 mL (same for both kits). Store at RT• Wash Solution 1 Concentrate, 1 x 16.4 mL. Store at RT• Wash Solution 2 Concentrate, 1 x 50 mL (same for both kits). Store at RT• Multi-Sample DNA Lysis Buffer, 1 x 55 mL (same for both kits). Store at RT• Nuclease-Free Water, 1 x 50 mL (same for both kits). Store at RT• PK Buffer, 1 x 11 mL (same for both kits). Store at RT• Processing Tubes, 50 tubes. Store at RT• Proteinase K, 1 x 900 μL. Store at -15° to -25°C• Ribonuclease A (RNase A), 1 x 290 μL. Store at -15° to -25°C
Isolation Technology
Magnetic Bead
Final Product Type
Genomic DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
MagMAX™
Quantity
50 Preps
Format
Kit
Sample Type
Blood, Buccal Samples, Cells, Tissue
For Use With (Application)
Genotyping, Real-Time Quantitative PCR (qPCR), PCR, Sequencing
No. of Reactions
50 Preps
Product Type
DNA Multi-Sample Kit
Target
Genomic DNA
Related Products
Description
- MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells
- These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis
- The 50 prep kit is designed for manual microfuge tube processing
- The demands for DNA yield and purity have become more stringent with the development of new analytical methods and technologies, such as high-throughput genotyping, and next-generation DNA sequencing platforms
- MagMAX™ DNA Multi-Sample Kits meet the challenge by delivering highly pure DNA that is free of nucleases, proteins, and other inhibitors of downstream enzymatic reactions
- As shown in Table 1, DNA with high yield and purity can be obtained from a variety of materials using the MagMAX™ DNA Multi-Sample Kits
- This is in contrast to filter membrane–based methods that often require different kit configurations for various input materials
- For applications requiring maximal yields, for small batch sizes, or for DNA archival purposes MagMAX™ DNA Multi-Sample Kit (tube format, manual processing) may be desirable because larger sample inputs are accommodated
- DNA yields suitable for most routine applications may be obtained using the MagMAX™-96 DNA Multi-Sample Kits (96-well plate format, compatible with automated handling) (Table 1)
- The MagMAX™ DNA Multi-Sample Kit also delivers high yields of DNA from lipid or glycogen rich samples, such as brain and liver tissues, respectively
- Figure 1 shows more than twice as much DNA was purified from tissues with the MagMAX™ DNA Multi-Sample Kit than with the leading competitor’s filter membrane–based method
- Most enzymatic molecular applications are prone to inhibition by analytes carried over from the sample matrix (e.g., heme, heparin, salts, protein) or the purification method itself (phenol, salts, alcohol)
- Figure 2 illustrates the effect of DNA purity on sensitive molecular applications, such as quantitative PCR (qPCR)
- DNA was extracted from blood using the MagMAX™-96 DNA Multi-Sample Kit, or a competitor’s filter membrane–based kit and subjected to real-time PCR analysis
- The cycle threshold CT values obtained with the DNA purified using the MagMAX™ DNA Multi-Sample Kit were lower than those obtained with the filter membrane kit–purified DNA, indicating the MagMAX™ kit yields higher-purity DNA
- MagMAX™ DNA Multi-Sample Kits provide purified DNA that is suitable for a variety of Applied Biosystems application workflows (Figure 3), including SNP genotyping and DNA sequencing analysis (see Figure 4)
- To see the full performance description of DNA purified using the MagMAX™ DNA Multi-Sample Kits in TaqMan™ SNP genotyping assays and with BigDye™ chemistry in DNA sequence analysis, view the MagMAX™ DNA Multi-Sample Kit Product Bulletin.