Applied Biosystems™ ExoSAP-IT™ExpressPCR Product Cleanup

Description

• Key features of ExoSAP-IT™ Express reagent: 5 minute protocol —Fastest turnaround time to results One-tube, one-step PCR cleanup —Add ExoSAP-IT Express directly to PCR product Novel enzyme technology —Irreversibly inactivated in just one minute at 80°C Conserve PCR samples —100% recovery of PCR products, regardless of amplicon length Eliminate spin columns or beads —Significantly decreases time and errors while increasing yield Compatible —No interference in downstream applications Scalable —Treat a wide range of volumes Multiple formats —Single tube, 8 tube strip, and 96-well plate formats offer flexibility from manual pipetting to automated workflows Go green —Generate less waste with a single-tube solution to PCR cleanup Overview It is necessary to remove excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing
• An enzymatic cleanup method offers precision and speed over technologies such as spin columns or beads
• ExoSAP-IT Express reagent consists of two hydrolytic enzymes, a modified Exonuclease I and Shrimp Alkaline Phosphatase (SAP), in a specially formulated buffer
• It is used to remove excess primers and dNTPs from a PCR mixture with no interference in downstream applications
• This unique, highly stable, one tube solution allows for greater confidence and superior sequencing results (Fig
• 1)
• Innovative solution Our scientists engineered Exonuclease I to have increased heat lability, allowing for inactivation in only 1 minute (Fig
• 2)
• ExoSAP-IT Express reagent is added directly to the PCR product for a 4 minute cleanup at 37°C, followed by a 1 minute inactivation at 80°C, which provides accurate and consistent results in just 5 minutes
• Fastest PCR cleanup method This novel technology allows for a significant reduction in sample cleanup time with minimal steps, providing the simplest workflow (Fig
• 3)
• ExoSAP-IT Express reagent offers rapid turnaround times and improved efficiency of resources while delivering the quality that scientists have become accustomed to when using ExoSAP-IT for PCR product cleanup
• Conserve PCR samples — simple one step, 100% recovery The ExoSAP-IT Express enzymatic cleanup method is designed to minimize errors by reducing your protocol to a single pipette step, allowing for automated or manual processing from a single tube or microtiter well
• ExoSAP-IT Express reagent outperforms the competition by ensuring 100% recovery of all amplicon sizes, both short and long (Table 1)
• ExoSAP-IT Express PCR Product Cleanup is active in commonly used PCR buffers, so no buffer exchange is required
• After treatment, ExoSAP-IT Express reagent is inactivated by heating at 80°C for 1 minute
• The treated PCR products are then ready for subsequent analysis in applications that require DNA to be free of excess primers and nucleotides.