Applied Biosystems™ MagMAX™ Cell-Free DNA Isolation Kit

Description

• The MagMAX™ Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) and optimized for use with biological samples such as serum and plasma
• The kit is based on MagMAX™ magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing
• Scalable isolation of circulating nucleic acid from plasma, serum, and urine cfDNA recovery is challenging due to the limited amount present in cell-free samples
• The MagMAX Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows you to easily process a wide range of sample input volumes, from 100 μL to 10 mL
• When used with either a KingFisher™ Duo Prime or KingFisher™ Flex magnetic particle processor, 6 to 24 plasma/serum samples of 2 mL input volume can be isolated at once
• Alternatively, samples can be processed manually with the use of a magnetic stand
• Features of the MagMAX Cell-Free DNA Isolation Kit include: • Flexible design that permits both manual and automated isolation of cfDNA • Scalable format that allows for higher and lower input volumes • Automation-ready, phenol-free extraction • Fast procedure, which allows 6 to 24 samples to be processed typically in 40 minutes or less when used with the KingFisher Duo Prime Magnetic Particle Processor or the KingFisher Flex Magnetic Particle Processor with a 24 Deep-Well Head • Wide sample volume inputs range from 500 μL to 10 mL of plasma/serum or urine • Contains Dynabeads™ MyOne™ Silane for consistent isolation of cfDNA • Elution volumes range from 15 μL to 50 μL Unlock a wealth of information with cell-free DNA Isolating and analyzing circulating cfDNA from plasma, serum, or urine has the potential to serve as a non-invasive approach to detect and monitor targets associated with certain disease states such as cancer
• The first step in attaining this valuable information is efficient nucleic acid isolation that specifically recovers the circulating cfDNA while leaving the larger DNA molecules behind
• This helps ensure that the shorter, fragmented DNA is concentrated and ready for downstream analysis using real-time PCR, digital PCR, or next-generation sequencing
• Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating cfDNA
• Beads bind the DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields
• Additionally, because filters and vacuum manifolds are not used, there is no risk of cellular particulates clogging these items during the extraction process
• This clogging issue is of particular concern with protein-rich, large-volume samples such as plasma that are commonly used in cfDNA studies
• Optimized surface chemistry The Dynabeads MyOne Silane contained in the MagMAX Cell-Free DNA Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture
• These aspects make Dynabeads MyOne Silane ideal for the recovery of circulating cfDNA targets present at low concentrations in cell-free body fluids
• Once captured, these rare DNA fragments can then be eluted in small elution volumes of 15 μL to 50 μL and are ready for downstream applications.