Applied Biosystems™ MagMAX™ DNA Ultra 2.0 with Cell and Tissue Extraction Buffer

Description

• The resulting purified gDNA is ideal for a variety of downstream molecular biology applications such as qPCR, next-generation sequencing, and microarray analysis
• One kit is sufficient to process 200 x 50–200 μL samples or 100 x 200–400 μL samples
• This version of the MagMAX DNA Multi-Sample Ultra 2.0 Kit contains an additional buffer that allows you to extract DNA from tissues and cells as well as blood, saliva, buffy coat, and buccal swabs
• Key features of the MagMAX DNA Multi-Sample Ultra 2.0 Kit: • Process 6–96 samples in 45 minutes with 5 minutes hands-on time • Isolate gDNA from 50 μL–2 mL of sample using a simple protocol in SBS microtiter plates • Skip sample and buffer volume normalization steps for hassle-free processing • Download validated BindIt software automation scripts for KingFisher Duo Prime and Flex purification systems • Automation-friendly stand-alone MagMAX DNA Multi-Sample Ultra 2.0 reagent bottle options available Isolate genomic DNA from multiple sample types with a single sample prep kit The MagMAX DNA Multi-Sample Ultra 2.0 Kit isolates gDNA from 50 μL–2 mL of whole blood (figure below), fresh and stabilized saliva (figure below), and EDTA/Streck cfDNA tube buffy coat (figure below) samples
• Additionally, the same kit can be used to isolate gDNA from buccal swabs and other biological sample types
• The MagMAX DNA Multi-Sample Ultra 2.0 Kit maximizes gDNA yields by treating samples with proteinase K (PK) and PK-enhancer followed by an innovative lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on the high-binding capacity Ultra 2.0 gDNA magnetic binding beads
• The Ultra 2.0 Wash I solution removes proteins, detergents, and other impurities from the bound nucleic acid
• The gDNA is then released from the beads using a low-TE elution solution
• Fast and hassle-free MagMAX magnetic bead-based gDNA workflows The MagMAX DNA Multi-Sample Ultra 2.0 Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort traditionally required by other methods without compromising yield and purity
• Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep
• Simply match your sample volumes to one of three standard input workflows (50 μL to 400 μL, up to 1 mL, up to 2 mL) and follow the work instructions for either manual or semi-automated sample preparation
• Made for KingFisher purification systems The MagMAX DNA Multi-Sample Ultra 2.0 Kit is optimized for use on the moderate-throughput KingFisher Duo Prime (Cat
• No
• 5400110) and the higher-throughput KingFisher Flex (Cat
• No
• 5400630) purification systems
• Users can process up to 400 μL of sample volume in high-throughput SBS 96-well deep-well microtiter plates using the Flex system
• For smaller sample batches, users can process 12 samples using the Duo Prime system
• Larger sample input volumes (1–2 mL), which are more challenging to process, are purified in SBS 24-well deep-well microtiter plates for either 24 samples on the Flex or 6 samples on the Duo Prime system
• KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments.