Invitrogen™ ChargeSwitch™ gDNA Normalized Buccal Cell Kit

Description

• The ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from human buccal swabs in less than 15 minutes after lysate preparation
• • Optimized for higher-purity DNA extraction • Ideal for STR profiling • Compatible with high-throughput protocols Rapid Purification Protocol The ChargeSwitch™ gDNA Normalized Buccal Cell Kit allows purification of genomic DNA from human buccal cell swabs or pelleted cells from a mouthwash in less than 15 minutes after lysates preparation
• ChargeSwitch™ gDNA Normalized Buccal Cell Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification
• High-quality genomic DNA is purified at a normalized concentration of 1-3 ng/μL in a total volume of 150 μL (Figure 1) by eliminating the need for lengthy quantitation and dilution steps
• Samples can be stored for up to 2 weeks at 4°C before processing without a noticeable loss in DNA yield or quality
• Note: DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used
• Validated for Single Tandem Repeat (STR) Profiling After employing the ChargeSwitch™ gDNA Normalized Buccal Cell Kit, purified gDNA is suitable for use in STR analysis of poor or environmentally degraded samples, and for downstream applications such as PCR
• Compatible with Single Tube and High-Throughput Protocols The ChargeSwitch™ gDNA Normalized Buccal Cell Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette
• For processing up to 24 individual samples at a time the MagnaRack™ (Cat
• No
• CS15000) two-piece magnetic separation tube rack is required
• This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch™ products
• For high-throughput applications utilizing 96 well blocks, the 96-well Magnetic Separator (Cat
• No
• CS15096) is required
• This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station
• For Research Use Only
• Not intended for diagnostic procedures
• Order Info Shipping Condition: Room Temperature