Invitrogen™ PureLink™ Genomic Plant DNA Purification Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K183001-Each-of-1 | In Stock | ₹ 25,839.12 |
K183001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 25,839.12
GST (18%): ₹ 4,651.042
Total Price: ₹ 30,490.162
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
gDNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
Up to 14.6 μg
Content And Storage
• 12.5 mL Resuspension Buffer R2; room temperature• 5 mL Precipitation Buffer N2; room temperature• 7.5 mL Binding Buffer B4; room temperature• 25 mL Wash Buffer W4; room temperature• 10 mL Wash Buffer W5; room temperature• 10 mL Elution Buffer; room temperature• 0.75 mL 20% SDS; room temperature• 0.75 mL RNase A; room temperature• 50 PureLink Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes; room temperature
Sample Type
Plants
Elution Volume
100 to 200 μL
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
50 Preps
Test Time
40 min.
Starting Material Amount
100 mg
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Description
- Description The PureLink Genomic Plant DNA Purification Kit allows total DNA purification from a variety of plant samples such as spinach, tomato, soy, wheat, Arabidopsis , mushroom, alfalfa sprouts, sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass, and Arabidopsis thaliana leaves in under one hour
- Efficient—Yields high quality DNA with minimal RNA contamination Simple DNA Purification from Plant Samples PureLink Genomic Plant DNA Purification Kit is suitable for isolating DNA from chloroplasts and variety of plant tissues Entire protocol relies on centrifugation of spin columns in absence of organic solvents PureLink Genomic Plant DNA Purification Kit is designed to purify DNA from up to 100 mg of plant tissue Maximize Yields of Total DNA High yield of DNA and minimal DNA degradation is achieved by using young plant samples such as leaves and by freezing sample in liquid nitrogen immediately after collection Total DNA is eluted in two aliquots of 100μL each to obtain greater DNA yield, with first elution generating between 65-80% DNA recovery and the second elution > 90% Typically, DNA isolated using the PureLink Genomic Plant DNA Purification Kit has an A 260 /A 280 > 1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications Isolated DNA can be used for a Number of Downstream Applications: Total DNA purification from different plant tissue with the PureLink Genomic Plant DNA Purification Kit is suitable for a variety of applications, including PCR, restriction enzyme digestion, and southern blotting