Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K210007-Each-of-1 | In Stock | ₹ 57,838.56 |
K210007 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,838.56
GST (18%): ₹ 10,410.941
Total Price: ₹ 68,249.501
Content And Storage
• 250 mL Resuspension Buffer (R3)• 1.5 mL RNase A• 250 mL Lysis Buffer (L7)• 250 mL Precipitation Buffer (N3)• 2 x 400 mL Equilibration Buffer (EQ1)• 3 x 500 mL Wash Buffer (W8)• 400 mL Elution Buffer (E4)• 30 mL TE Buffer• 25 HiPure Columns• 5 Column HoldersStore all components at room temperature.
Isolation Technology
Anion Exchange Resin
Scale
Maxi
Final Product Type
BAC DNA, Plasmid DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Product Line
PureLink™
Quantity
25 Preps
Shipping Condition
Room Temperature
System Type
PureLink™
Yield
1000 μg
Format
Column
Sample Type
Bacterial Culture
Elution Volume
15 mL
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions
25 Preps
Prep Scale
> 200 μg (Large-Scale) Plasmid DNA
Product Type
Plasmid MaxiPrep Kit
Purity
Transfection Grade
Target
BAC DNA, Plasmid DNA
Test Time
2 hr.
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Description
- Description The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E
- coli
- The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients
- Labor and time saving- no need for extra steps to remove endotoxin and other contaminants High-purity, low-endotoxin product- pure enough for mammalian cell transfection Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs Anion-exchange chromatography for plasmid purification PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal Plasmid preparations can typically be completed in less than 2 hours Contaminant-free DNA Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A 260 /A 280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection Purification time is two hours Order Info Shipping Condition: Room Temperature