Invitrogen™ PureLink™ProQuick96 Plasmid Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K211024A-Each-of-1 | In Stock | ₹ 5,56,995.60 |
K211024A - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,56,995.60
GST (18%): ₹ 1,00,259.208
Total Price: ₹ 6,57,254.808
Content And Storage
The PureLink™ Pro Quick96 Plasmid Kit includes PureLink™ Pro Quick96 Resuspension Buffer, Lysis Buffer, Neutralization Buffer, RNase A, Wash Buffers, Elution Buffer, square-well blocks, Quick96 Filtration Plates, Quick96 Binding Plates, wash plates, elution plates, and a manual. Store components at room temperature. Store Resuspension Buffer after addition of RNase A at +4°C. Guaranteed stable for one year when properly stored.
Isolation Technology
Silica Plate
Elution Volume
50 μL (centrifuge), 100 μL (vacuum), 150 µ:L
For Use With (Equipment)
Biomek™ FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual)
Product Line
PureLink™
Quantity
24 x 96 Preps
Target
Plasmid DNA
Test Time
45 min.
Starting Material Amount
1.2-1.5 ml lysate⁄well
Format
96-well Plate
Sample Type
Bacterial Culture
Final Product Type
Plasmid DNA
For Use With (Application)
Next-Generation Sequencing, Sequencing, PCR, Cloning
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Type
Plasmid Purification Kit
Purity
Molecular Biology Grade
System Type
PureLink™
Yield
15 μg
Related Products
Description
- The PureLink™ Pro Quick96 Plasmid Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E
- coli in as little as 45 minutes (Table 1)
- Using the PureLink™ Pro Quick96 Plasmid Kit, you will: • Obtain consistently high yields of high-purity plasmid DNA • Experience ease of use and better performance due to improved plate design and protocol • Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms How it works The PureLink™ Pro Quick96 Plasmid Kit uses two 96-well plates, a filtration plate (clear O-ring), and a binding plate (red O-ring) to purify plasmid DNA
- Bacterial cells are subjected to alkaline lysis and are pelleted by centrifugation
- The bacterial lysate is then applied to the Quick96 Filtration Plate for clearing and collection in the Quick96 Binding Plate, where the plasmid DNA binds to the silica membrane
- After washing steps to remove contaminants, purified DNA is eluted and ready for use in downstream applications
- The entire procedure can be completed in 45 minutes (Figure 1)
- Improved plate design The PureLink™ Pro Quick96 Plasmid Kit provides optimized semiskirted 96-well plates for improved performance and results (Figure 2)
- The Quick96 Filtration Plate is designed for fast and efficient clearing of the bacterial lysates
- The Quick96 Binding Plate offers an increased binding capacity of up to 20 μg plasmid DNA
- In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover
- In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform
- The semiskirted plate design is compatible with most vacuum manifolds on robotic workstations
- Higher yields and purity The PureLink™ Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20 μg of plasmid DNA from E
- coli strains grown in up to 5 mL LB, 3 mL 2xYT, or 3 mL TB
- In an automated platform using 1.5 mL E
- coli culture grown overnight in a square-well block, typical yields are 5-15 μg plasmid DNA, with an average of 9.4 μg (Figure 3)
- The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA (Figure 4)
- The PureLink™ Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning
- Order Info Shipping Condition: Room Temperature