Invitrogen™ Champion™ pET200 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically CompetentE. coli
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K20001-Each-of-1 | In Stock | ₹ 96,340.56 |
K20001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 96,340.56
GST (18%): ₹ 17,341.301
Total Price: ₹ 1,13,681.861
Constitutive or Inducible System
Inducible
Promoter
T7, lacO
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Kanamycin (KanR)
Cleavage
EK (Enterokinase) Recognition Site
Expression Mechanism
Cell-Based Expression
Cloning Method
Directional TOPO
Vector
pET
Inducing Agent
IPTG
Product Type
TOPO Expression Kit
Content And Storage
Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competentE. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain
BL21 Star™(DE3)
Expression System
E. coli
Protein Tag
His Tag (6x), Xpress Epitope Tag
Quantity
20 reactions
Product Line
One Shot™
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Description
- Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because the T7 RNA polymerase is more processive than native E
- coli RNA polymerase and is dedicated to transcription of gene of interest Protein production is further enhanced in system by expression strain BL21 Star E
- coli , which significantly improves stability of mRNA transcripts and increases protein expression up to ten-fold Simplified, efficient Directional TOPO cloning allows to quickly enter a ChampionpET Expression vector These kits feature linearized, topoisomerase I-activated ChampionpET expression vectors for five-minute directional cloning to facilitate gene expression with proofreading enzyme used for PCR, resulting in fewer errors in cloned genes Greater than 90% of clones are in correct orientation for gene expression, reducing the time spent on colony screening Bacterial ExpressionProtein ExpressionProteins, Expression, Isolation and Analysis