K601001
Invitrogen™ Flp-In™ Complete System
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K601001-Each-of-1 | In Stock | ₹ 1,98,499.20 |
K601001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,98,499.20
GST (18%): ₹ 35,729.856
Total Price: ₹ 2,34,229.056
Constitutive or Inducible System
Constitutive
Promoter
CMV
Selection Agent (Eukaryotic)
Zeocin™, Hygromycin
Protein Tag
Untagged
Quantity
1 kit
Product Line
Flp-In™, pcDNA™
Delivery Type
Transfection
Product Type
Flp-In Complete System
Content And Storage
The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored.
Cloning Method
Restriction Enzyme/MCS
Vector
pFRT
For Use With (Application)
Stable Cell Line Development, Targeted Integration
Related Products
Description
- Introduce of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site Major components include Flp-In
- target site vector, pFRT/lacZeo, for generation of host cell line containing integrated FRT site Expression plasmid containing a FRT site linked to hygromycin resistance gene for Flp recombinase-mediated integration and selection of stable cell line expressing gene of interest under control of human cytomegalovirus (CMV) immediate-early enhancer/promoter Flp recombinase expression plasmid, pOG44, for expression of Flp recombinase under control of human CMV promoter Control expression plasmid containing chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into the Flp-In
- host cell line, expresses CAT Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Stable Cell Line Development, Targeted Integration