PR-A1380
Promega pGEM™-T and pGEM™-T Easy Vector Systems
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PR-A1380-Each-of-1 | In Stock | ₹ 50,651.52 |
PR-A1380 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,651.52
GST (18%): ₹ 9,117.274
Total Price: ₹ 59,768.794
Promoter
SP6, T7
Content And Storage
<-65°C
Vector
pGEM-T easy vector II
Restriction Site
BstZI, NotI and EcoRI
Quantity
20 Reactions
For Use With (Application)
For the routine subcloning of PCR products
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Description
- The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with Eco R V and adding a 3« terminal thymidine to both ends
- These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid
- 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide f1 Origin of Replication allows the preparation of single-stranded DNA pGEM-T Vector Systems Multiple cloning site is flanked by recognition sites for the restriction enzyme Bst Z I Single-enzyme digestion allows release of the insert Double digestion may also be used to release the insert from the vector pGEM-T Easy Vector Systems Recognition sites for Bst Z I, Eco R I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion