V801001

Invitrogen™ pIZT/V5-His Vector Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V801001-Each-of-1 In Stock ₹ 1,02,843.12

V801001 - Each of 1

₹ 1,02,843.12

In Stock

Quantity

1

Base Price: ₹ 1,02,843.12

GST (18%): ₹ 18,511.762

Total Price: ₹ 1,21,354.882

Promoter

OplE2

Content And Storage

The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilizedOpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Protein Tag

His Tag (6x), V5 Epitope Tag

Quantity

1 kit

Product Line

InsectSelect™

Product Type

Insect Cell Expression Vector

Protein Tag Position (to your gene)

C-terminal

Cloning Method

Restriction Enzyme/MCS

Vector

pIZ

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Description

  • Description The OpIE 2 promoter for constitutive expression The Zeocin resistance gene for rapid selection of stably transfected cell lines C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP) The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells Constitutive Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis