A65393
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A65393-Each-of-1 | In Stock | ₹ 31,657.20 |
A65393 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,657.20
GST (18%): ₹ 5,698.296
Total Price: ₹ 37,355.496
Product Type
DNA Amplification Kit
For Use With (Application)
RCA, MDA-WGA
Quantity
100 Reactions
Content And Storage
• EquiPhi29 DNA Polymerase (0.1 mL at 10 U/μL)• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1 mL)• 0.1 M DTT (0.25 mL)• 500 μM Exo-Resistant Random Primer (0.2 mL)• 10 mM dNTP Mix (0.2 mL)• 0.1 U/μL Pyrophosphatase, inorganic (0.1 mL)Store at -15 to -25°C.
Optimal Reaction Temperature
42°C
Reaction Time
Up to 2 hours
Form
Liquid
Polymerase
EquiPhi29 DNA Polymerase
Research Category
Nucleic Acid Analysis,In VitroTranscription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Format
Kit
Shipping Condition
Dry Ice
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Description
- The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA)
- Features of the EquiPhi29 DNA Amplification Kit include: • Fast DNA amplification in less than two hours • Sensitivity from 1 fg of input DNA • Maximal yield of 17 µg amplified DNA • Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies) • Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression • All required components for DNA amplification in RCA or MDA-WGA reactions The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias
- Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs
- The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.