Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Description

• Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions
• Lyo-ready RPA reagents are available in stand-alone and kit formats
• Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets
• All reagents are glycerol-free, which enables assay lyophilization
• Features of Lyo-ready RPA products Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer Robust and specific RNA/DNA amplification Amplification from inhibitor-containing samples Fast target amplification in as little as 20 minutes Ability to easily optimize RPA or RT-RPA reactions Kit components The Lyo-ready RPA Kit includes all required components for an RPA reaction: Lyo-ready T4 UvsX Protein —produced from E
• coli cells bearing cloned uvsX gene derived from bacteriophage T4 Lyo-ready T4 UvsY Protein —a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase Lyo-ready Bst DNA Polymerase (Cat
• No
• A56655) —a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors Lyo-ready T4 Gene 32 Protein —a single-stranded DNA binding protein produced from E
• coli cells carrying cloned bacteriophage T4 gene 32 Optimized reaction buffer, magnesium chloride, and dNTPs