Invitrogen™mirVana™ PARIS™ RNA and Native Protein Purification Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1556-Each-of-1 | In Stock | ₹ 41,496.60 |
AM1556 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,496.60
GST (18%): ₹ 7,469.388
Total Price: ₹ 48,965.988
Content And Storage
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 25 mL Cell Disruption Buffer (4°C)• 25 mL 2X Denaturing Solution (4°C)• 2 × 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time
30 min.
Final Product Type
Total RNA, Transcriptome RNA, siRNA, Protein (Native), snRNA, micro RNA
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Western Blotting, microRNA Analysis, Northern Blotting, cDNA Library Construction
Product Line
mirVana™, Ambion™
Starting Material Amount
Up to 10^7 cells, Up to 100 mg tissue
Isolation Technology
Organic Extraction, Cell Lysis (Nonionic Detergent), Spin Column (Glass Fiber Filter)
Sample Type
Cell Cultures, Tissue (Mammalian)
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
40 Preps
Shipping Condition
Room Temperature
Target
Total RNA, RNA and Protein, siRNA, miRNA
Related Products
Description
- The mir Vana™ PARIS™ Kit is for the purification of both native protein and RNA (including small RNA) from the same sample
- Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolations of protein and separate large and small RNA fractions
- How the kit works The mir Vana PARIS Kit procedure begins with homogenization of samples with a special cell disruption buffer that includes nonionic detergent
- Protein remains intact, so a portion of the lysate can be used directly for common protein analysis applications
- The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both
- Isolate small RNA-containing total RNA and native protein from the same sample Start with 100–10 7 cultured cells or up to 100 mg of many mammalian tissues Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses Follow a simple, 30-minute procedure Use to correlate mRNA, miRNA or siRNA, and protein levels Not high-throughput compatible (Manual) Order Info Shipping Condition: Room temperature