Invitrogen™ PureLink™ Total RNA Blood Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K156001-Each-of-1 | In Stock | ₹ 39,186.48 |
K156001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 39,186.48
GST (18%): ₹ 7,053.566
Total Price: ₹ 46,240.046
Content And Storage
• 17.5 mL Lysis Buffer L3; store at room temperature• 175 mL Lysis Buffer L5; room temperature• 60 mL Wash Buffer W4; room temperature• 10 mL Wash Buffer W5; room temperature• 5 mL RNase-free Water; room temperature• 50 Spin Cartridges with Collection Tubes; room temperature• 50 Wash Tubes (2 mL); room temperature• 50 Elution Tubes (1.7 mL); room temperature
Purification Time
20 min. (After sample homogenization)
Sample Type
Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 preps
Shipping Condition
Room Temperature
Yield
1000 μg (Binding capacity)
Isolation Technology
Silica Spin Column
Elution Volume
30–100 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale
Mini
Starting Material Amount
Blood: ≤500 μL
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Description
- PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol
- Simple RNA Purification from Whole Blood PureLink Total RNA Blood Kit is designed for purifying from 50 to 500 μL fresh whole blood or approximately 3.5 x 10 6 leukocytes (white blood cells) in less than 1 hour
- The entire protocol can be done in spin tubes and spin columns
- Leukocyte Enrichment for Improved RNA Purification Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm 3 ) and leukocytes, with the latter present in the minority (5000 - 7000/mm 3 )
- Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns
- The distinct feature of the PureLink Total RNA Blood Kit is that it employs a leukocyte enrichment step
- Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes
- Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications
- Consequently, yields of high-quality total RNA are obtained using the PureLink Total RNA Blood Kit
- RNA purification from whole blood in under an hour Yields high-quality RNA devoid of non-nucleated erythrocytes Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products: Northern Blotting Kits & Reagents Nuclease protection assays Microarray cDNA synthesis Real-Time PCR (qPCR) Not high-throughput compatible (Manual) Applications DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood Order Info Shipping Condition: Room temperature